Enzymes that catalyse the ligation of two molecules with concomitant
hydrolysis of the pyrophosphate bond in adenosine 5'-triphosphate (ATP) or a similar triphosphate,
forming
C–C
,
C–O
,
C–S
,
P–O
or
C–N
bonds.
In
recombinant DNA technology,
ligases covalently join together two sequences of
DNA (e.g.
hostDNA and foreign
DNA) by a phosphodiester bond.
Source:
PAC, 1992, 64, 143
(Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992))
on page 159
Cite as:
IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by
A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997).
XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic,
J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8.
https://doi.org/10.1351/goldbook.
