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complementary DNA (cDNA)

A single-stranded DNA molecule with a nucleotidesequence that is complementary to an RNA molecule; cDNA is formed by the action of the enzyme reverse transcriptase on an RNAtemplate. After conversion to the double-stranded form, cDNA is used for molecular cloning or for hybridization studies.
Source:
PAC, 1992, 64, 143 (Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992)) on page 150
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IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8. https://doi.org/10.1351/goldbook.
Last update: 2014-02-24; version: 2.3.3.
DOI of this term: https://doi.org/10.1351/goldbook.C01202.
Original PDF version: http://www.iupac.org/goldbook/C01202.pdf. The PDF version is out of date and is provided for reference purposes only. For some entries, the PDF version may be unavailable.
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