A highly concentrated analyte in an
analytical sample can be determined with better
precision by replacing the
blank (reference) cell by one containing a solution of the analyte or other
absorber of known concentration; this is known as difference absorption
spectroscopy. Difference spectra can also be obtained by computer or other subtraction methods.
Source:
PAC, 1988, 60, 1449
(Nomenclature, symbols, units and their usage in spectrochemical analysis - VII. Molecular
absorption spectroscopy, ultraviolet and visible (UV/VIS) (Recommendations 1988))
on page 1455